Breast cancer is the leading cause of cancer death in females, and approximately 75% are estrogen receptor (ER)-positive breast tumors. Tamoxifen (TAM) has been a frontline treatment for both early and advanced ER-positive breast cancer in pre- and postmenopausal women. TAM is a selective ER modulator and its active metabolite, 4-hydroxytamoxifen (4-OHT), acts as an estrogen antagonist in breast cells that binds ER and blocks its activity to halt cell proliferation and induce apoptosis. Unfortunately, TAM resistance occurs in approximately 30% of patients. Additionally the development of side effects with long-term administration, including menopausal symptoms, fatigue, painful joints and mood changes, significantly affects quality of life and often leads to discontinuation of treatment. TAM in combination with other therapies is being actively investigated as a way to increase efficacy and decrease side effects. Numerous studies have demonstrated that natural compounds present in vegetables, fruits and mushrooms can affect multiple molecular targets and signaling pathways, leading to their possible use in combination therapy. BreastDefend® (BD) is a dietary supplement formula which contains a combination of medicinal mushrooms (Ganoderma lucidum, Coriolus versicolor, Phellinus linteus), herbal extracts (Curcuma longa, Scutellaria barbata, Astragalus membranaceus), and purified biologically active components (3, 3′-diindolylmethane, quercetin). These natural agents have demonstrated anticancer activities against breast cancer in multiple studies. Prior studies on BD alone or combined with modified citrus pectin (MCP) showed significant inhibition of growth and invasive behavior of highly metastatic triple-negative human breast cancer cells in vitro and in vivo…
I’m grateful for the opportunity to present its internationally conference on cancer research 2019 the topic of my presentation today is breast defend enhances effect of tamoxifen in estrogen receptor-positive human breast cancer in vitro and individual the paper was published in 2017 at bmc complementary and alternative medicine breast cancer is the most common
Cancer in women which affects one in six one in seven women and we all are aware of the devastating effect of breast cancer in principle if we had to divide breast cancer into two basic groups they will be divided into homan insensitive as present positive estrogen and progesterone positive and estrogen and progesterone negative often triple negative or here two
New positive but as present negative and progesterone negative and the treatments for these cancers post initial chemotherapy are very different women who estrogen positive have the ability of getting treated with hormonal blockades of different kind either receptor and therapies such as tamoxifen or aromatase inhibitors while there is a clear distinction when it
Comes to this therapeutic strategy between triple negative and estrogen positive breast cancer when it comes to the realm of natural ingredients and the ability to combine them together we have a possibility that the same formulation will be effective for both triple negative breast cancer and estrogen positive breast cancer in this sense this formulation breast
Defend is very unique prior to the paper that i’m presenting today we have published a number of papers on this formula in these papers we have shown that breast defend was able to inhibit in vitro and in vivo both triple negative breast cancer with high metastatic potential as well as estrogen positive breast cancer breast defend was able to significantly reduce
The metastatic potential in metastasis to the lung from triple negative breast cancer that was implanted in the mammary paint of mice breast defend includes a number of ingredients it includes coot eliab a beta ban gillian tumeric rhizoma kakuma astragalus as well as quercetin it also includes a proprietary blend of medicinal mushrooms coriolis reishi and filly
Nose what’s unique about these mushrooms is that they are grown specifically on immune enhancing and anti-cancer botanicals so the mushrooms are being nourished and grown on these herbs and as a result the immune enhancing and anti-cancer properties of these mushrooms can be enhanced it also includes di m d indole methane it is significant milligram dose what is
Interesting is that some of these ingredients specifically for example di m have shown to have effect on both triple negative as well and estimate it as well as estrogen positive more than this di m for example was shown to reduce the expression of praça in double-blind clinical trials so when we put such a formula together the idea is to enhance the effects by
Creating a synergistic effect and also to provide some compound that can reduce the side effects of conventional treatments so this is a logic behind press defense we have shown in previous papers that by combining such ingredients we can reach an effective in vitro and in vivo effect it much lower dosages compared to using a single ingredient in the specific
Paper that i’m presenting today we compared the antiproliferative effects of breast defend to tamoxifen and the potential synergistic effect in inhibiting the growth of mcf7 estrogen positive breast cancer that was implanted into the memory pet of the mice mcf-7 cell lines were treated both in vitro as well as in vivo in addition we analyzed the tumors in the
Breast defend group in the tamoxifen group and in the combination group compared to controlled to see that any specific change in gene expression between these groups once the tumors established the mice were randomly divided into four groups of 13 animals each control group press defend tamoxifen in combination of tamoxifen and precision the key assessment in
Response in the in vivo group was changes in tumor volume compared to the control group breast defend and tamoxifen had similar effects in reducing the growth of the primary tumors compared to control however when breast defending tamoxifen were combined there was a significant reduction in tumor volume compared to breast defend all tamoxifen alone the results of
The combination of tamoxifen and press defend on the regulation of expression of apoptosis related proteins we looked at broth p21 bcl-2 in fibronectin while tamoxifen had a very small effect on increasing the expression of p21 in oncogenes suppression protein breast defend enhanced it much more significantly and the combination had a similar effect looking at
Bcl-2 which enhances the aggressiveness of the tumor in the metastatic potential breast defend and tamoxifen reduced and decreased the expression of bcl-2 and there was a synergistic effect and when we looked at the effect on fibronectin there was a clear mystic effect when tamoxifen and press defend were used together freedo two shows the effects of breast defend
Tamoxifen and the combination on tumor volume it clearly shows a synergistic effect when we are using breast defend an tamoxifen together to summarize in this study we have demonstrated synergistic effects between tamoxifen and breast defend and we also demonstrated the effects on protein expression that can explain the synergistic effects and the benefit of this
Combination in the past we’ve already shown the ability of this formula to significantly reduce the metastatic potential and metastatic tendency in triple negative breast cancer cell line in vitro and in vivo and we also now have some preliminary pre published data showing the possibility of this formulation to prevent occurrence of triple negative breast cancer
In animals in mice with tendency for spontaneous expression of triple negative breast cancer the next step which is very natural is the movement into clinical trials we are now in the final design in submission of two clinical trial that will test this formulation both in estrogen positive breast cancer patients between diagnosis and surgery and in triple negative
Breast cancer post first-line therapy where there was residual tumor in the surgical tissues that was removed but there were clear margins we hope that with these studies we can establish more accurately the role of this formulation how to optimize it in how to use it with other therapies i’m grateful again for the opportunity to present the discoveries thank you
Transcribed from video
Breastdefend Enhances Effect of Tamoxifen in Estrogen Receptor-Positive… By ICCR 2019
